HIGH SPEED CAMERA
frame rate up to 2000 fps
HIGH SENSITIVITY
down to single photon level
LONG TERM STREAMING
record directly to hard drive
Description
The HiCAM Fluo is a high-speed camera for fluorescence imaging applications. It records high resolution images at a frame rate of up to 2000 fps in the most challenging low-light conditions by using a cooled image intensifier. Packed into a compact aluminum enclosure, it is easy to attach the HiCAM Fluo to any fluorescence microscope.
Fluorescence Imaging Applications
High-speed fluorescence imaging, bioluminescence and chemiluminescence detection for in-vivo imaging
Time-resolved imaging of fluids for microfluidic research
Particle Image Velocimetry (PIV)
Time-resolved imaging and spectroscopy using ultra-short exposures
Laser-Induced Fluorescence (LIF)
Super-slow motion combustion research
Plasma physics research
Single-photon imaging for astronomy
Features
Fiber-optically coupled intensifier
This offers a unique combination of high speed imaging and increased light sensitivity down to single photon level.
CoaXPress
To transfer all the high-resolution image data, the HiCAM Fluo streams live over a CoaXPress (CXP) interface. The camera has four CXP connectors, each of which has a channel speed of 6.25 Gbit/s.
Ultra-Short Gating
With its gated image intensifier, the camera’s effective exposure time can be reduced. The minimum gate width of 10 ns (FWHM) increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering.
Cooled Image Intensifier
The fanless design of the camera minimises vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Fluorescence Imaging Videos
Recording of a beating heart of a zebrafish. Recorded at 2000 fps with the Lambert Instruments HiCAM on a fluorescence microscope. The blood cells were stained with a DS-red fluorophore.
monArch fluorescent voltage indicator observed with the Lambert Instruments HiCAM Fluo (1000 fps, 1200 x 1000 pixels).
Specifications
HICAM FLUO, 1000 FPS
Maximum resolution: 1280 x 1024 pixels
Frame rate:
1000 fps at full resolution
1500 fps at 1200 x 720 pixels
4000 fps at 640 x 480 pixels
7500 fps at 256 x 256 pixels
Pixel size: 6.6 um
Pixel depth: 8 or 12 bit
Minimum exposure time: 40 ns
Gating repetition rate: 100 kHz
Image intensifier: GaAsP (Others optional)
Computer interface: Streaming CoaXPress
HICAM FLUO, 2000 FPS
Maximum resolution: 1920 x 1080 pixels
Frame rate:
2000 fps at full resolution
2500 fps at 1280 x 960 pixels
5100 fps at 640 x 480 pixels
6400 fps at 512 x 384 pixels
Pixel size: 10 um
Pixel depth: 8 bit
Minimum exposure time: 40 ns
Gating repetition rate: 100 kHz
Image intensifier: GaAsP (Others optional)
Computer interface: Streaming CoaXPress 2.0
Request more information
Lambert Instruments BV
Leonard Springerlaan 19
9727 KB Groningen
The Netherlands
User Publications
Researchers around the world use the HiCAM Fluo in their studies. Opposite is an overview of the most recent publications describing research that was done using a HiCAM Fluo.
For a complete overview of applications, please visit our applications pages.
