VERSATILE
available in Widefield, Spinning-disk confocal, and TIRF configurations
AFFORDABLE
the most cost-effective way of doing quantitative FLIM
COMPATABLE
LIFA is compatible with every fluorescence microscope with a camera output
Description
The Lambert Instruments LIFA FLIM system is the fastest and easiest way to perform Fluorescence Lifetime Imaging Microscopy (FLIM).
Available in versatile configurations dependent on your specific applications, the LIFA system offers a turn-key solution for fluorescence lifetime imaging microscopy. Compatible with any fluorescence microscope with a camera output – including microscopes by Leica, Nikon, Olympus, TILL and Zeiss. Set up is quick and easy, with all hardware integrated seamlessly with our dedicated LIFA software, so you can focus on your experiment.
The advanced software instantly analyses data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph, while detailed statisitcal data can be exported to an Excel worksheet.
Read more about LIFA software.
Features
Experiments from start to finish
From recording images to lifetime calculation and data analysis
Dedicated LIFA software
Instantly calculates the fluorescence lifetime and presents it as a colour coded overlay
Broad Lifetime Range
From sub microsecond down to picoseconds
Multiple configurations
System components to suit your applications
Non-phototoxic illumination
Applications
Live-cell Imaging
Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.
This video shows a time-lapse of HeLa cells. After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime.
FLIM data courtesy of the Netherlands Cancer Institute.
Bacteria research
B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime.
Image courtesy of the University of Groningen.
High-throughput screening
Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.
Image courtesy of the University of Groningen.
LIFA vTAU
SPAD powered FLIM system
Simplify experiments for researchers and imaging centres with the vTAU SPAD camera; combining excellent light sensitivity with easy image acquisition and data analysis.
Minimise measurement duration, automate image acquisition, and simplify data analysis… factors of great importance for cell biology, cancer research and high-throughput screening.
vTAU easily integrates into any FLIM system, providing a plug-n-play experience that allows for seamless switching between setups.
Key features
Unique image sensor
Ultra-high sensitive SPAD detector for up to 100 lifetime images per second
Multiple image modes
Including regular Frequency-Domain FLIM acquisition and Time-Lapse recordings
Characteristics
Pixel resolution: 512 x 512 px
Pixel size: 16 μm
Lifetime range: 0.2 to 300ns
Frame rate: up to 300 fps
Sensor type: SPAD
Downloads
System Components
The LIFA vTAU system includes:
Dedicated LIFA Software
Light source(s) according to your application
Computer with USB connection
On-site system installation
1 day of hands-on training
Phone, email and remote desktop support
LIFA Software
Seamless integration of hardware for full system control
Guides user through FLIM experiments from start to finish.
Supports third-party hardware for a flexible and expandable system.
Light sources
Multi-LED and/or Multi-LASER as required for your set up
Excitation light sources for frequency-domain fluorescence lifetime imaging, supplied according to your required wavelengths.
Configurations
Widefield
On widefield microscopes, the Toggel camera in combination with the Multi-LED offers a capable yet compact FLIM solution. The Toggel is compatible with the camera port of widefield microscopes and the Multi-LED is compatible with the standard epifluorescence port of widefield microscopes.
Spinning-disk confocal
Being a camera-based system, the Lambert Instruments LIFA system for frequency-domain FLIM is compatible with multi-beam confocal microscopy techniques, most notably the Yokogawa CSU spinning disk series (based on the Nipkow disk scanner), and the VTInfinity series by Visitech International.
TIRF
Request More Information
Lambert Instruments BV
Leonard Springerlaan 19
9727 KB Groningen
The Netherlands
User Publications
Researchers around the world use the LIFA in their studies. Opposite is an overview of the most recent publications describing research that was done using a LIFA.
For a complete overview of applications, please visit our applications pages and our selected LIFA publications on the Lambert main site for more information.
Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic β-catenin
July 19 – FLIM
Electrocatalytic Reaction Driven Flow: Role of pH in Flow Reversal
Mar 2, 2022 – FLIM, proton concentration, University of Twente
Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
Dec 7, 2021 – Microscopy, Fluorescence
Quantification of PD-1/PD-L1 Interaction between Membranes from PBMCs and Melanoma Samples Using Cell Membrane Microarray and Time-Resolved Förster Resonance Energy Transfer
Dec 7, 2021 – FRET, Fluorescence
Glycerol suppresses glucose consumption in trypanosomes through metabolic contest
Aug 13, 2021 – FLIM
PIM kinases inhibit AMPK activation and promote tumorigenicity by phosphorylating LKB1
Jun 30, 2021 – FLIM
Featured LIFA users
