Fluorescence imaging is a technique used in various fields of science, including biology, chemistry, and materials science, to visualise and study the distribution and behaviour of fluorescent molecules within a sample. This technique relies on the property of certain substances to absorb light at one wavelength and then re-emit it at a longer wavelength, which is visible light. These substances are called fluorophores or fluorescent dyes.

Applications of fluorescence imaging are diverse and include cell biology, microbiology, neuroscience, genetics, cancer research, and materials science. It is commonly used in techniques like fluorescence microscopy, flow cytometry, and fluorescence in situ hybridisation (FISH) to investigate the localisation, interactions, and dynamics of biological molecules and structures.