How should I start up and shut down the setup?

On startup, first make sure all the hardware cables (power and USB) are connected. Then turn on the LIFA control unit, and start up the LI-FLIM software.

For shutdown first close the LI-FLIM software and then turn off the LIFA control unit. Make sure that when you are done, you switch the microscope port from camera port back to eyepiece, to prevent unwanted light reaching the intensified camera. Turn off the mains power.