I switched on the camera but I don’t see anything

THERE IS NO SIGNAL TO THE CAMERA

Is the microscope switched to the camera port?

Do you see light through the eyepiece when switching to eyepiece?

Are you using the right fluorescence filter cube?

Is there a shutter, neutral density (ND) filter or field stop in the light path? (the LIFA Multi-LED has a shutter, is it open?)

Did you press the ‘FLIM’ button to put the system into live mode?

Is the LED/laser on?

THE SIGNAL IS LOW

What is your MCP setting? Please test with MCP values up to around 800V.

Did you use a long enough exposure time (i.e. >100 milliseconds)?

Are you using the right fluorescence filter cube?

Are you using ND filters, please put the system in ‘IDLE’ mode and remove them, if any.

THERE IS NO LIGHT ON MY SAMPLE

Are you using the right excitation filter?

Is there a shutter, neutral density (ND) filter or field stop in the light path? (the LIFA Multi-LED has a shutter, is it open?)

Did you press the ‘FLIM’ button to put the system into live mode?

Is the LED/laser on?