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with SPAD powered vTAU camera
The complete high-speed recording solution
High-speed camera for fluorescence imaging
Intensified high-speed camera
Intensified camera with ultra-short gating
High-speed Intensified Camera Attachment
Compact lens-coupled image intensifier
Intensifier Control
Unit for Automated Systems
Record and edit high-speed videos with one or multiple cameras.
Record and analyze fluorescence lifetime images.
Custom imaging products, sensors and software for low light level applications.

The calculated lifetimes are different from what I expected

Are you using the right fluorescence filter cube?

Are you using the right reference solution and reference lifetime for calibrating the system? Did you change anything in the optical setup between taking a Reference and a Sample? Please note that each optical setup requires its own reference calibration; i.e. switching dichroics, filters, objectives, light source, magnifications, or power light levels changes the path lengths inside the system. The same holds when using a different MCP gain for the intensified CCD camera. Changing ND filters or the exposure time does not require a different reference calibration.

Is there reason to believe that the (photophysics of) your sample is as expected?

Is there auto-fluorescence influencing the fluorescence lifetime distribution?

In case your application is FRET; is there leak-through from your acceptor in the donor channel?

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