< Back
with SPAD powered vTAU camera
The complete high-speed recording solution
High-speed camera for fluorescence imaging
Intensified high-speed camera
Intensified camera with ultra-short gating
High-speed Intensified Camera Attachment
Compact lens-coupled image intensifier
Intensifier Control
Unit for Automated Systems
Record and edit high-speed videos with one or multiple cameras.
Record and analyze fluorescence lifetime images.
Custom imaging products, sensors and software for low light level applications.

Frequency-Domain FLIM: Basic Equations

In this article the first principles of frequency domain (FD) fluorescence lifetime imaging microscopy (FLIM) are further explained through use of equations. Although these principles not necessary for the execution of basic lifetime measurements, a thorough understanding provides the groundwork that enables deeper insight into your results and into the possibilities of FD lifetime imaging.

Fluorescence Lifetime 

For an ensemble of fluorescent molecules the rate at which molecules decay from the excited state to the ground state (cf. Fig.1) is proportional to the number of excited state molecules N:

Homodyne Detection

Contact Us

5th floor,
Leonard Springerlaan 19
9727KB Groningen
The Netherlands

Phone:
+31 (0) 50 501 8461
Email:
sales@lambertinstruments.com