The Lambert Instruments LIFA FLIM system is the fastest and easiest way to perform Fluorescence Lifetime Imaging Microscopy (FLIM).
Available in versatile configurations dependent on your specific applications, the LIFA system offers a turn-key solution for fluorescence lifetime imaging microscopy. Compatible with any fluorescence microscope with a camera output – including microscopes by Leica, Nikon, Olympus, TILL and Zeiss. Set up is quick and easy, with all hardware integrated seamlessly with our dedicated LIFA software, so you can focus on your experiment.
The advanced software instantly analyses data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph, while detailed statisitcal data can be exported to an Excel worksheet.
Read more about LIFA software | Watch our FLIM webinar.
Captured with LIFA vTAU: Dual-color, high-speed 3D imaging of blood flow in a beating embryonic zebrafish heart.
Experiments from start to finish
From recording images to lifetime calculation and data analysis
Using our stand-alone LIFA Software, you can instantly calculate the fluorescence lifetime and presents it as a colour coded overlay and a phaser plot.
Broad Lifetime Range
From sub microsecond down to picoseconds
Our software development kit (SDK) provides a flexible platform for integrating and automating experiments, offering you full control and customization of your fluorescence lifetime measurements.
Multiple configurations
System components to suit your applications
Non-phototoxic illumination
Widefield
The Lambert Instruments VTAU camera, when combined with the Multi-LED, provides a compact and effective solution for FLIM on widefield microscopes.
The VTAU easily connects to the camera port, while the Multi-LED integrates with the standard epifluorescence port, making the setup compatible with most widefield microscope configurations.
Spinning-disk confocal
The Lambert Instruments LIFA VTAU system for frequency-domain FLIM is well-suited for camera-based multi-beam confocal techniques.
It is compatible with systems such as the Yokogawa CSU series (Nipkow disk), Andor Dragonfly, Crest V3/V2/Cicero, and Visitech International’s VTInfinity series, enabling versatile imaging performance.
TIRF
Total Internal Reflection Fluorescence (TIRF) microscopy enables high-contrast fluorescence imaging close to the cover glass, typically within a ~100 nm optical section.
When combined with frequency-domain FLIM, it allows for lifetime imaging of structures such as membrane receptors, aiding in the analysis of cellular signalisation pathways.
Seamless integration of hardware for full system control
Multi-LED and/or Multi-LASER as required for your set up
We offer an OEM platform based on the proven Lambert Instruments LIFA system
Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique that measures the decay time of fluorescence molecules, so-called fluorophores. FLIM provides detailed information about molecular environments, energy transfer between fluorophores and protein interactions. It is widely used in biological and biomedical research to study dynamic processes and cellular microenvironments.
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique that measures the fluorescence lifetime of molecules by exciting fluorophores with a very short pulse of light and then detecting the time it takes for them to emit photons. The emitted light’s intensity decays over time, and this decay curve is analyzed to determine the fluorescence lifetime.
Frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique used to measure the fluorescence lifetimes of fluorophores by modulating the excitation light at different frequencies. In this method, the phase shift and modulation depth of the emitted light relative to the excitation source are measured, which provides information about the fluorescence lifetime. This technique is valuable for studying molecular interactions, environment-sensitive probes, and cellular dynamics. FLIM is commonly used in biological and biomedical imaging, to sense the cellular environment, get a better understanding of cell biology or to differentiate between fluorophores based on their lifetimes if they have similar emission spectra.
The primary difference between frequency-domain and time-domain FLIM lies in how the fluorescence lifetime is measured:
Frequency-domain FLIM: Modulates the excitation light at specific frequencies and measures the phase shift and modulation of the emitted light relative to the excitation. It determines lifetime from these shifts.
Time-domain FLIM: Uses short light pulses to excite fluorophores and measures the time delay between the excitation pulse and the emitted photons. The fluorescence decay curve is analyzed to determine lifetime.
Both techniques provide insights into molecular environments but differ in how they capture fluorescence data.
Widefield microscopy is an imaging technique used in biological and clinical research to capture images of entire specimens or large sections of tissue. It involves illuminating the entire field of view at once with light, typically from a broad light source, such as a lamp or LED. All parts of the sample are illuminated simultaneously, allowing for the detection of light emitted or reflected from the entire specimen. This method is commonly used for fluorescence microscopy, enabling researchers to observe labeled structures in live or fixed cells.
Spinning-disk confocal microscopy is an advanced imaging technique used for capturing high-resolution images of biological samples in real-time. It employs a rotating disk with multiple pinholes to focus light onto specific areas of the sample while rejecting out-of-focus light. This enables faster image acquisition compared to traditional laser-scanning confocal microscopy. Spinning-disk confocal is ideal for live-cell imaging due to its speed, lower phototoxicity, and reduced photobleaching, making it suitable for dynamic processes like cell movement or protein interactions.
Total Internal Reflection Fluorescence (TIRF) microscopy is a technique used to observe events occurring near the surface of a cell, typically at or near the plasma membrane. TIRF systems use an evanescent wave generated by light undergoing total internal reflection at the interface between two media (e.g., glass and water) to selectively illuminate and excite fluorophores within a very thin region, typically less than 200 nm, adjacent to the surface. This minimizes background noise and allows high-contrast imaging of cellular processes like protein interactions at the cell membrane.
Light sheet microscopy, also known as Selective Plane Illumination Microscopy (SPIM), is a technique used to image large, three-dimensional biological samples with minimal phototoxicity and photobleaching. In this method, a thin sheet of light illuminates a single plane of the sample, while an orthogonal detection system captures high-resolution images. By moving the light sheet through the sample, researchers can reconstruct detailed 3D images of tissues, embryos, or entire organisms. This method is especially useful for long-term live imaging of biological processes in real-time.
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10-20 times more light efficiency in a compact and light weight form
choose from a wide variety of high sensitivity image intensifiers to match the needs of your application
high sensitivity supplemented with an acquisition speed up to 160 fps.
The TRiCAM is a compact intensified low-light imaging CMOS camera. It is designed for scientific and industrial applications that require low-light imaging. With built-in signal generators, the TRiCAM is capable of ultra-short exposures through fast gating and therefore suitable for time-resolved imaging.
Laser Induced Fluorescence (LIF)
Time-gated luminescence
Bio- and chemiluminescence imaging
Plasma physics
Single Photon imaging
Particle Image Velocimetry (PIV)
Solar PV and LED characterization
Combustion research
Single-molecule imaging
Fluorescence Lifetime-Imaging
Microscopy (FLIM)
Förster Resonance Energy Transfer
(FRET)
Time-gated Raman / Laser Induced
Breakdown Spectroscopy (LIBS)
Time-resolved imaging & spectroscopy
Diffuse Optical Tomography (DOT)
High voltage Partial Breakdown (Partial discharge)
Gated image intensifier
The TRiCAM is equipped with an integrated timing pulse generator and a gate-unit, generating gate pulses down to < 3 ns.
Low-light imaging
The TRiCAM has a built-in image intensifier that boosts the incoming light. This way, you can capture detailed images in the most challenging light conditions.
Fiber-optically coupled
Our experienced engineers couple the sensor to the image intensifier with a fiber-optic plate, resulting in a 10 – 20x more light efficient system. The fiber-optic plate is a solid piece of glass that consists of millions of parallel glass fibers sealed together. Each fiber acts as an independent light conductor that transfers the light from the image intensifier to the sensor.
Ultra-short gating
The image intensifier in the TRiCAM can be used as an ultra-fast electronic shutter. This technique is called gating and it can be done in a matter of nanoseconds.
Gating can eliminate motion blur when imaging fast-moving objects or highly dynamic processes. By varying the timing of the gate signal very precisely (tens of picosecond resolution), you can use gating to record a time-resolved light intensity profile.
Optimised for your application
The TRiCAM can be configured with a wide range of image intensifiers. Available image intensifiers cover the entire visual spectrum and the near infrared.
High-resolution sensor
The TRiCAM features a high-resolution CMOS sensor. It captures stunningly detailed images at 1920 x 1200 pixels.
160 fps
At up to 160 fps, the TRiCAM can record slow-motion footage, even in low-light conditions.
Global shutter
With its global shutter readout method, the sensor in the TRiCAM eliminates rolling shutter effects in your images.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Low-light imging
The TRiCAM has a built-in image intensifier that boosts the incoming light. This way, you can capture detailed images in the most challenging light conditions.
Our experienced engineers will help you pick the right image intensifier for your application.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Diameter: 18 mm
Minimum Gate Width: 40 ns (< 3 ns optional)
Maximum Repetition Frequency: 300 kHz
Trigger Input: TTL
Resolution: 1920 x 1200 pixels
Pixel Size: 5.86 µm
Frame Rate: 162 fps
Sensor Type: CMOS
Readout Method: Global Shutter
ADC: 10 bit and 12 bit
For more information on the TRiCAM CMOS Camera, please download the brochure.
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Researchers around the world use the TRiCAM in their studies. Opposite is an overview of the most recent publications describing research that was done using a TRiCAM.
For a complete overview of applications, please visit our applications pages.
and possibility to request for custom firmware
Computer controlled by USB connection
Seamlessly integrates with Lambert Instruments’ intensifier attachments
For integration into a larger system or for automated measurements, the gain control unit for automated systems lets you control the settings of the intensifier attachment. The control unit uses standard TTL and analog signals for communication, allowing the user to switch the intensifier on or off, alter the gain and the anode current limit of the image intensifier without the need of software integration.
Time resolved imaging
Synchronization imaging
Rapid Gate switching to improve dynamic range
To be used with Lambert Instruments’ Intensifier Attachments.
Image Intensifier Gain
By increasing the image intensifier gain, the incoming light intensity will be boosted more, resulting in a brighter image. The intensifier control for automated systems lets you control the gain of the image intensifier.
Anode Current Limit
To protect the fragile image intensifier from being damaged by overexposure, the anode current limiter can be used to set a limit for the acceptable anode current. If the anode current exceeds this value, then the image intensifier will be switched to a safe mode.
Compatibility
The gain control unit for automated systems is compatible with our intensifier attachments: HiCATT and TRiCATT
A synchronisation signal is needed in order to create an output at the three outputs of the intensifier gate control. This signal can originate from different sources:
External source: For instance a signal from a frame grabber or a connected camera.
Software command
Internal sync source: Gives synchronisation pulses with a fixed frequency. Can generate pulses at frequencies between 0.5 Hz and 4 MHz.
When using an external synchronisation source, the following modes are available:
– Synchronise with rising edge of external input pulse
– Synchronise with falling edge of external input pulse
– Follow external input pulse
| Timing Resolution | 250ps |
|---|---|
| Jitter Pulse Width | <250 ps RMS |
| Jitter Delay | <250 ps RMS |
| Sync with falling/rising edge | Follow external output post | |
|---|---|---|
| Pulse width /delay range | 10ns – 10s | 10ns – 10s |
| Timing resolution (Step size) | 10ns | 10ns |
| Jitter pulse width | <10ps RMS | <10ps RMS |
| Jitter delay | <10ns RMS | <250ns RMS |
| Insertion delay | 30ns | 20ns |
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for low light imaging with increased sensitivity
adjust for changing light levels by cycling through three different gate widths
outer-body focus ring adjusts the internal relay optics without altering total length
The High-speed Intensified Camera Attachment (HiCATT) is designed for use with a high-speed camera. It increases the sensitivity of your camera and enables low-light imaging at frame rates up to 1 MHz (10 Mhz in burst)
The technology in the HiCATT expands the dynamic range of your high-speed camera. At low light levels, even single photons can be detected. While at high light levels, overexposure can be prevented by very short exposures (down to 3 ns). These short exposures yield sharp images of fast moving objects.
Combustion research / Hydrogen discovery
High-speed fluorescence, bioluminescence and chemiluminescence detection for in-vivo imaging
Super-slow motion combustion research for the automotive industry
Time-resolved imaging in plasma physics research
Dynamic phenomena in microscopy
Laser-Induced Fluorescence (LIF)
Many other industrial or scientific low-light-level applications in high-speed imaging
UV imaging for hydrogen and fusion
The HiCATT is an intensifier attachment for your high-speed camera. It provides increased light sensitivity for high-speed imaging applications. In this photo, the HiCATT is attached to a Phantom Veo 410S.
Optimised for your application
The HiCATT can be configured with a wide range of image intensifiers. Our experienced engineers will help you pick the right image intensifier for your application.
High-speed Imaging
The HiCATT upgrades your high-speed camera to the next level of performance. It boosts the intensity of incoming light at speeds up to 1,000,000 fps.
Ultra-short exposures
The gated image intensifier enables exposure times down to 3 ns. At such short exposure times, motion blur is eliminated completely to ensure sharp images.
50% QE high-sensitivity intensifiers
You can choose from a wide variety of high-sensitivity image intensifiers to match the spectral needs of your application.
Rotating camera mount
Safe and easy camera coupling keeping the HiCATT and the camera in their intended orientation.
Compatible with Shimadzu and Vision Research – We can provide fully integrated solutions tailored to your setup.
Match my camera
The relay optics of HiCATT and TRiCATT project the output of the image intensifier onto the sensor of your camera. This calculator helps you determine the optimal configuration of image intensifer diameter and relay optics for your camera and application.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Phosphors
The output of the image intensifier contains a layer of phosphorescent material. Upon impact of an electron, the phosphor screen will emit light. Depending on the type of phosphor, the intensity of the emitted light will decrease faster.
Butane-Propane Flame at 4200 FPS
Flames (mix Butane – Propane) at 4200 fps and 40 us gate open time (effective exposure time), HiCATT 25 image intensifier, high-speed camera attachment with Phantom V4.0 high-speed camera.
Electronic Discharge at 47000 FPS
Electronic Discharge at 47000 fps and 3 us gate open time (effective exposure time), HiCATT 25 image intensifier, high-speed camera attachment with Phantom V7.1 high-speed camera.
Gunshot at 15000 FPS
Non-Intensified vs. Intensified recording of a gunshot with a Phantom V7.1 high-speed camera. Part 1: recorded at 15000 fps and 61µs gate open time (effective exposure time). Part 2: recorded at 15000 fps and 2µs gate open time.
Gas Combustion at 5000 FPS
Gas combustion observed at 5,000 fps with HICATT High Speed Image Intensifier, Gen 2, 10µs exposure time. The HICATT High Speed Intensifier used for this video was coupled to a NAC Memrecam camera. It is also compatible with pco.Dimax, Phantom, Photron Fastcam or Optronis cameras.
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Researchers around the world use the HiCATT in their studies. Opposite is an overview of the most recent publications describing research that was done using a HiCATT.
For a complete overview of applications, please visit our applications pages.
Ultraviolet Laser Absorption Imaging of High-Speed Flows in a Shock Tube
May 22, 2023 Combustion
Mach 4 Flow Velocimetry with 100-kHz PLEET and PIV in AEDC/AFRL Tunnel D Jan 4, 2022
Simultaneous OH, CH2O and flow field imaging of near blowoff dynamics Jan 4, 2022
Meteorite Ablation and High-Speed Emission Spectra in Plasma Wind Tunnel Jan 4, 2022
Ultraviolet Laser Absorption Imaging of High-Speed Flows in a Shock Tube Jan 4, 2022
Lambert Instruments specialises in high-end imaging solutions and is known for producing Custom Imaging Products. Custom imaging products for low-light level applications can be made according to customer specifications.
These can be made according to customer specifications, such as special multi-stage intensifiers, cooled intensifiers, intensified high-speed cameras, intensified CCD cameras, intensified CMOS CCD cameras, and image sensors with fibre optic input windows.
Our standard product range offers only a part of the available types of camera sensors. But our custom solutions can include just about every type of sensor you can think of.
We have experience with
– Line-scan sensors
– Fiber-coupled scintillators
– Fiber-coupled line scan/TDI sensors
– Sensors with interchangeable fiber-optical window
-High speed spectral imaging
Lambert Instruments has over twenty years of experience with image intensifiers. Our engineers are experts in choosing the right image intensifier for your imaging application.
Our highly specialised customs products include
– Cooled image intensifiers for ultra-low noise
– Specialised image intensifier configurations for corona imaging in high-voltage applications
– Multi-stage image intensifiers with multiple boosters
Lambert Instruments offers custom software solutions for a wide variety of imaging standards, including
– GigE Vision
– GenICam
– CoaXPress
– CameraLink
For more information about our standard software solution, please visit our software page.
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available in Widefield, Spinning-disk confocal, and TIRF configurations as well as Spectral and SPIM
a cost-effective way of doing quantitative FLIM
LIFA is compatible with every type of fluorescence microscope with a camera output
The Lambert Instruments LIFA FLIM system is the fastest and easiest way to perform Fluorescence Lifetime Imaging Microscopy (FLIM).
Available in versatile configurations dependent on your specific applications, the LIFA system offers a turn-key solution for fluorescence lifetime imaging microscopy. Compatible with any fluorescence microscope with a camera output – including microscopes by Leica, Nikon, Olympus, TILL and Zeiss. Set up is quick and easy, with all hardware integrated seamlessly with our dedicated LIFA software, so you can focus on your experiment.
The advanced software instantly analyses data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph, while detailed statisitcal data can be exported to an Excel worksheet.
Read more about LIFA software.
Experiments from start to finish
From recording images to lifetime calculation and data analysis
Dedicated LIFA software
Using our stand-alone LIFA Software, you can instantly calculate the fluorescence lifetime and presents it as a colour coded overlay and a phaser plot.
Comprehensive SDK
Our software development kit (SDK) provides a flexible platform for integrating and automating experiments, offering you full control and customization of your fluorescence lifetime measurements.
Broad Lifetime Range
From sub microsecond down to picoseconds
Multiple configurations
System components to suit your applications
Non-phototoxic illumination
Bacteria research
B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime.
Image courtesy of the University of Groningen.
High-throughput screening
Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.
Image courtesy of the University of Amsterdam.
Live-cell Imaging
Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.
This video shows a time-lapse of HeLa cells. After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime.
FLIM data courtesy of the Netherlands Cancer Institute.
Simplify experiments for researchers and imaging centres with the vTAU SPAD camera; combining excellent light sensitivity with easy image acquisition and data analysis.
Minimise measurement duration, automate image acquisition, and simplify data analysis… factors of great importance for cell biology, cancer research and high-throughput screening.
vTAU easily integrates into any FLIM system, providing a plug-n-play experience that allows for seamless switching between setups.
Unique image sensor
Ultra-high sensitive SPAD detector for up to 74 FLIM images per second
Multiple image modes
Including regular Frequency-Domain FLIM acquisition and Time-Lapse recordings
Pixel resolution: 512 x 512 px
Pixel size: 16 μm
Lifetime range: 0.2 to 300ns
Frame rate: up to 370 fps
Sensor type: SPAD
Unlock a new dimension in fluorescence imaging with FLIM for Beginners. Unlike intensity-based methods, FLIM measures fluorescence decay—revealing insights into biochemical and biophysical environments. This guide covers the fundamentals, the two main approaches (Time and Frequency Domain), and Phasor Analysis, plus key applications like metabolic imaging, protein interactions, and fluorophore unmixing.
Download it to strengthen your FLIM knowledge and enhance research in cancer, neuroscience, and drug discovery.
Dedicated LIFA Software
Light source(s) according to your application
Computer with USB and HDMI connection
On-site system installation (optional)
1 day of hands-on training (optional)
Phone, email and remote desktop support
Seamless integration of hardware for full system control
Guides user through FLIM experiments from start to finish.
Supports third-party hardware for a flexible and expandable system.
Time-lapse capabilities for extended experiments.
Data export for seamless analysis and sharing.
Triggered recording for precise data capture.
SDK and API available for custom integrations.
Multi-LED and/or Multi-LASER as required for your set up
Excitation light sources for frequency-domain fluorescence lifetime imaging, supplied according to your required wavelengths.
For widefield illumination, we provide multi-LED solutions.
For laser illumination, we offer multi-LASER options with single-mode, multi-mode, and liquid light guide configurations.
Multi-LED systems can also be provided with a liquid light guide.
We offer an OEM platform based on the proven Lambert Instruments LIFA system
Designed for system integrators who need a camera-based FLIM solution with short time to market.
Our solution is compact, high-speed, and easy to integrate, giving you the flexibility to embed FLIM capabilities into your microscopy systems without compromise.
High temporal resolution delivers fast and accurate lifetime imaging.
Widefield
Spinning-disk confocal
TIRF
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Researchers around the world use the LIFA in their studies. Opposite is an overview of the most recent publications describing research that was done using a LIFA.
For a complete overview of applications, please visit our applications pages and our selected LIFA publications on the Lambert main site for more information.
A green lifetime biosensor for calcium that remains bright over its full dynamic range Jan 02, 2025 – FLIM
Experimental variables determine the outcome of RAS-RAS interactions Oct 05, 2024 – FLIM
Identification of an H-Ras nanocluster disrupting peptide July 09 2024 – FLIM
Fluorescence lifetime multiplexing with fluorogen activating protein FAST variants July 02, 2024 – FLIM
Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic β-catenin July 19, 2023 – FLIM
Frequently Asked Questions
Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique that measures the decay time of fluorescence molecules, so-called fluorophores. FLIM provides detailed information about molecular environments, energy transfer between fluorophores and protein interactions. It is widely used in biological and biomedical research to study dynamic processes and cellular microenvironments.
Frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique used to measure the fluorescence lifetimes of fluorophores by modulating the excitation light at different frequencies. In this method, the phase shift and modulation depth of the emitted light relative to the excitation source are measured, which provides information about the fluorescence lifetime. This technique is valuable for studying molecular interactions, environment-sensitive probes, and cellular dynamics. FLIM is commonly used in biological and biomedical imaging, to sense the cellular environment, get a better understanding of cell biology or to differentiate between fluorophores based on their lifetimes if they have similar emission spectra.
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique that measures the fluorescence lifetime of molecules by exciting fluorophores with a very short pulse of light and then detecting the time it takes for them to emit photons. The emitted light’s intensity decays over time, and this decay curve is analyzed to determine the fluorescence lifetime.
The primary difference between frequency-domain and time-domain FLIM lies in how the fluorescence lifetime is measured:
Both techniques provide insights into molecular environments but differ in how they capture fluorescence data.
Widefield microscopy is an imaging technique used in biological and clinical research to capture images of entire specimens or large sections of tissue. It involves illuminating the entire field of view at once with light, typically from a broad light source, such as a lamp or LED. All parts of the sample are illuminated simultaneously, allowing for the detection of light emitted or reflected from the entire specimen. This method is commonly used for fluorescence microscopy, enabling researchers to observe labeled structures in live or fixed cells.
SPAD (Single-Photon Avalanche Diode) detector technology is used to detect individual photons with extremely high sensitivity and precision. SPADs operate in “Geiger mode,” where a single photon triggers an avalanche of charge carriers, resulting in a detectable electrical pulse. This allows for the precise detection of very low light levels, making SPAD detectors ideal for applications like fluorescence lifetime imaging microscopy (FLIM), quantum cryptography, time-of-flight measurements, and biomedical imaging. SPADs offer fast response times and high photon detection efficiency, enabling high-resolution and accurate photon counting.
Spinning-disk confocal microscopy is an advanced imaging technique used for capturing high-resolution images of biological samples in real-time. It employs a rotating disk with multiple pinholes to focus light onto specific areas of the sample while rejecting out-of-focus light. This enables faster image acquisition compared to traditional laser-scanning confocal microscopy. Spinning-disk confocal is ideal for live-cell imaging due to its speed, lower phototoxicity, and reduced photobleaching, making it suitable for dynamic processes like cell movement or protein interactions.
Light sheet microscopy, also known as Selective Plane Illumination Microscopy (SPIM), is a technique used to image large, three-dimensional biological samples with minimal phototoxicity and photobleaching. In this method, a thin sheet of light illuminates a single plane of the sample, while an orthogonal detection system captures high-resolution images. By moving the light sheet through the sample, researchers can reconstruct detailed 3D images of tissues, embryos, or entire organisms. This method is especially useful for long-term live imaging of biological processes in real-time.
Total Internal Reflection Fluorescence (TIRF) microscopy is a technique used to observe events occurring near the surface of a cell, typically at or near the plasma membrane. TIRF systems use an evanescent wave generated by light undergoing total internal reflection at the interface between two media (e.g., glass and water) to selectively illuminate and excite fluorophores within a very thin region, typically less than 200 nm, adjacent to the surface. This minimizes background noise and allows high-contrast imaging of cellular processes like protein interactions at the cell membrane.
Gen II and Gen III image intensifiers offering the world’s highest resolution and sensitivity in the UV, visible or near infrared
outer-body focus ring adjusts the internal relay optics without altering total length
easily fits within your imaging or spectroscopy setup, with a super-compact 18mm version also available
The TRiCATT is a compact lens-coupled image intensifier for scientific and industrial applications that require:
– Low-light level imaging
– Ultra-short exposures through fast gating
Any camera with C-mount and a 1/2″, 2/3″, or 1″ image sensor is compatible with the TRiCATT. You can find the right TRiCATT for your camera with our interactive calculator.
Time-resolved imaging and spectroscopy
Particle Image Velocimetry (PIV)
Laser Induced Fluorescence (LIF)
Diffuse Optical Tomography (DOT)
Time-gated luminescence
Forster Resonance Energy Transfer (FRET)
Oxygen imaging
Viscosity imaging
Single-molecule imaging
Bio- and chemiluminescence imaging
Solar PV and LED characterization
Combustion research
Time-gated Raman
Plasma physics
X-ray Imaging
Single photon imaging
Small gate widths
Gate width down to less than 3 ns (FWHM) with minimal jitter.
High gate repetition widths
Up to 300 kHz / 2.5 MHz burst. 1Mhz Optional
Overexposure Protection
User-definable current limitation and optional shutter.
Easy Coupling
Efficient lens coupling to any CCD and CMOS camera (up to 500 fps) with C-mount input and output.
Relay Lens
The high quality relay lens transfers the intensified image to the image sensor of the attached camera very efficiently and without losses in resolution. If required we can provide the 0.5x relay lens with a back focal distance of 13 mm.
Camera
Along with the Image Intensifier TRiCATT Lambert Instruments can deliver different types of CCD and CMOS cameras. If you already have a camera, you can use our interactive calculator to determine which intensifier size and relay optics are best suited for your setup.
Match my camera
The relay optics of HiCATT and TRiCATT project the output of the image intensifier onto the sensor of your camera. This calculator helps you determine the optimal configuration of image intensifer diameter and relay optics for your camera and application.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Phosphors
The output of the image intensifier contains a layer of phosphorescent material. Upon impact of an electron, the phosphor screen will emit light. Depending on the type of phosphor, the intensity of the emitted light will decrease faster.
TRiCATT 25
Image intensifier: 25 mm image intensifier. Gated or modulated. P43 phosphor
Minimum gate time: 3 ns, 40 ns or 50 ns
Gate repetition rate*: 100 kHz, 300 kHz or 1 MHz
Relay optics**: 1:1 or 1.7:1 Fixed aperture
Input mount: F-mount and C-mount
Output mount: F-mount and C-mount
Mechanical shutter: Optional
Mounting plate: Optional
TRiCATT 18
Image intensifier: 18 mm image intensifier. Gated or modulated. P43 phosphor
Minimum gate time: 3 ns, 40 ns or 50 ns
Gate repetition rate*: 100 kHz, 300 kHz or 1 MHz
Relay optics**: 1:1 Fixed aperture
Input mount: F-mount and C-mount
Output mount: F-mount and C-mount
Mechanical shutter: Optional
Mounting plate: Optional
TRiCATT 18C
Image intensifier: 18 mm image intensifier. Gated or modulated. P43 phosphor
Minimum gate time: 3 ns, 40 ns or 50 ns
Gate repetition rate*: 100 kHz, 300 kHz or 1 MHz
Relay optics**: 1:1 Variable aperture
Input mount: C-mount
Output mount: C-mount
Mechanical shutter: Custom design upon request
Mounting plate: Optional
Standard Gating
Width range: 40 ns – 10 s
Resulting min. pulse width (increments): 40 ns (20ns)
Pulse repetition rate: < 10 MHz
Delay jitter (width): ± 10 ns (± 250 ps)
Insertion delay: 100 ns
Fast Gating
Width range: < 3 ns – 10 s
Resulting min. pulse width (increments): < 3 ns (10 ps)
Pulse repetition rate: < 16 MHz
Delay jitter (width): < 35 ps (< 35 ps)
Insertion delay: 20 ns
Trigger input: Programmable trigger level, divider and bursts (m out of n triggers)
The control unit contains a micro-controller, a high voltage power supply and a RF (Radio Frequency) amplifier. The control unit has a low voltage input to receive the external modulation signal. It amplifies this signal and biases it with a variable DC photocathode voltage. The control unit offers control of the MCP voltage for setting the image intensifier gain. The control unit also monitors the light output, and switches off the image intensifier when its light output becomes too high. The control unit supports modulation frequencies up to 120 MHz.
| Manual Gain Controller | Gain Control | Gate Control | Gate Generator | |
|---|---|---|---|---|
| Gain control | ✔ | |||
| Anode current limiter | ✔ | ✔ | ||
| Shutter control | ✔ | ✔ | ||
| Gain control | ✔ | ✔ | ✔ | |
| Gate control | ✔ | ✔ | ||
| Internal trigger generator | ✔ | ✔ | ||
| Programmable gate | ✔ |
Optional: Signal Generator
Instead of using an external modulation signal generator, we offer a built-in modulation signal generator as part of the control unit/power supply for frequencies up to 120 MHz.
Optional: TRiCAM
As an alternative to the lens-coupled ICCD camera (TRiCATT + CCD), we offer an ICCD camera in which the image intensifier is fiber-optically coupled to the sensor. This is the TRiCAM. This modulated intensified CCD camera is very compact and has a significantly higher gain than the lens-coupled combination as a result of the more efficient and compact fiber coupling.
Leonard Springerlaan 19
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Researchers around the world use the TRiCATT in their studies. Opposite is an overview of the most recent publications describing research that was done using a TRiCATT .
For a complete overview of applications, please visit our applications pages.
delivering unprecedented stamina, speed and sensitivity
single photon counting suitable for particle physics by making use of scintillation
ranging from Deep UV to NIR to match the spectral needs of your application
The HiCAM is a gated intensified high-speed high-sensitivity imaging camera. It has an integrated fiber-optically coupled image intensifier, which offers a unique combination of high speed and sensitivity down to single photon level. Because the HiCAM does not need high intensity light sources, it is suitable for use in low-light level conditions.
Super-slow motion combustion research for the automotive industry
Time-resolved imaging in plasma physics research
Dynamic phenomena in microscopy, e.g. Imaging the rotation of single molecules of ATPase
Laser-Induced Fluorescence (LIF)
Flow visualization and velocity measurements using Particle Image Velocimetry (PIV)
Time-resolved imaging of fluids for microfluidic research
Blood flow analysis
Time-resolved fluorescence
UV imaging for hydrogen combustion
Ultra-short Gating
The camera’s effective exposure time can be reduced to < 3 ns (FWHM) for time resolved imaging, or capturing very fast events.
Fast Streaming
To transfer all the high-resolution image data, the HiCAM streams live over a CoaXPress (CXP) interface. The camera has four CXP connectors, each of which has a channel speed of 6.25 Gbit/s.
Longterm Streaming
The recordings can be directly streamed to the hard drive. For optimised recorders the length is limited only by drive capacity.
High-sensitivity Intensifiers
You can choose from a wide variety of high-sensitivity image intensifiers to match the spectral needs of your application.
Cycled Bursts
Adjust for changing light levels by cycling through three different gate widths and optionally combine this with the burst mode to optimize the dynamic range.
Fiber-optically Coupled
Our experienced engineers couple the sensor to the image intensifier with a fiber-optic window.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Super-slow motion video of a gas flame recorded with the Lambert Instruments HiCAM at 1000 fps (frame rate) and gating of 15 us (effective exposure time). Resolution: 1280 x 512 pixels.
High-speed recording of a cork burning in plasma. Recorded with a HiCAM at 5000 fps.
Recording of a beating heart of a zebrafish. Recorded at 2000 fps with the HiCAM on a fluorescence microscope. The blood cells were stained with a DS-red fluoropho.
A touch-me-not plant bursting open. Captured with a HiCAM with 18 us gating.
Framerate (Full Resolution): 1000 fps
Sensor Resolution: 1280 x 1024 pixels
Streaming: HiCAM 1000: CoaXPress
Bit Depth: 8 and 12 bit
Framerate (Full Resolution): 2000 fps
Sensor Resolution: 1920 x 1080 pixels
Streaming: HiCAM 2000: CoaXPress
Bit Depth: 8
The dual-stage image intensifier, specially designed for high-speed cameras, can be equipped with a variety of photocathodes; ranging from ultra violet to infra red. Adapting the photocathode will give maximum output brightness to enhance Signal-to-Noise Ratio (SNR). Moreover, the image intensifier is fiber-optically coupled to a CMOS sensor. This further increases SNR, as compared to a lens-coupled system.
Leonard Springerlaan 19
9727 KB Groningen
The Netherlands
Researchers around the world use the HiCAM for high-speed high-sensitivity imaging in their studies. Opposite is an overview of the most recent publications describing research that was done using a HiCAM.
For a complete overview of applications, please visit our applications pages.
to optimise efficiency and reliability of your production process
without intermittent downloads
view and record images with multiple cameras at the same time.
Stamina combines advanced hardware with easy to use software to offer a complete multi-camera recording solution for your imaging challenges. With the complete imaging system it becomes possible to record, view and combine multiple cameras in one setup. Our software is compatible with a wide range of cameras, providing the option to integrate your own cameras into the imaging setup easily.
With Stamina, all recordings can be synchronised and all image data is directly streamed to the solid storage, enabling longterm streaming. With the external Application Programming Interface (API), Stamina can be further customised and integrated in your existing setup. Through the API, you have access to device settings, recording parameters and image data.
Stamina is a completely customizable multi-camera recording and imaging solution. Our experienced sales engineers will advise you on the right system for your application.
Troubleshooting high speed events (triggered recording)
Reliability engineering
Long-term multi camera recording
High speed / High resolution recording with multiple cameras
Add your Own Camera
Stamina is compatible with a wide range of cameras, providing the option to integrate your own (high-speed) cameras into the imaging setup easily.
Complete Solution
Stamina combines advanced hardware with easy to use software to offer a complete solution for your imaging challenges.
Long term streaming
By directly streaming to the hard drive, Stamina makes long term streaming easy. The maximal recording length only depends on the available storage space.
Control all hardware into one place
Change and control the settings of all compatible hardware in one place with Capture.
API Available
Integrate Stamina into your existing setup with the Application Programming Interface (API). The following platforms are compatible with the API: MATLAB, LabVIEW, Python 3 (64 bit).
Leonard Springerlaan 19
9727 KB Groningen
The Netherlands
Read about STAMINA’s launch at Photonics West 2022.
frame rate over 2000 fps
down to single photon level
record directly to hard drive
The HiCAM Fluo is a high-speed camera for fluorescence imaging applications. It records high resolution images at a frame rate over 2000 fps in the most challenging low-light conditions by using a cooled image intensifier. Packed into a compact aluminum enclosure, it is easy to attach the HiCAM Fluo to any fluorescence microscope.
High-speed fluorescence imaging, bioluminescence and chemiluminescence detection for in-vivo imaging
Time-resolved imaging of fluids for microfluidic research
Particle Image Velocimetry (PIV)
Time-resolved imaging and spectroscopy using ultra-short exposures (<3ns)
Laser-Induced Fluorescence (LIF)
Super-slow motion combustion research
Plasma physics research
· Single-photon imaging microscopy and quantum physics
Plasma physics research for fusion energy
Fiber-optically coupled intensifier
This offers a unique combination of high speed imaging and increased light sensitivity down to single photon level.
CoaXPress
To transfer all high-resolution image data, the HiCAM Fluo streams live over a CoaXPress (CXP) interface. The camera features four CXP connectors, each supporting channel speeds of up to 6.25 Gbit/s (CXP-6). For higher bandwidth requirements, CXP-12 compatibility ensures even faster data transfer, optimizing performance for demanding imaging applications.
Ultra-Short Gating
With its gated image intensifier, the camera’s effective exposure time can be reduced. The minimum gate width of 10 ns (FWHM) increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering.
Cooled Image Intensifier
The fanless design of the camera minimises vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras.
Image Intensifiers
An image intensifier boosts the intensity of the incoming light. By converting photons to electrons and back to photons, the light intensity can be increased significantly. Scroll down for more information about how this works.
Another feature of an image intensifier is that it can act as an ultra-fast shutter. The photocathode can be switched between on and off in a matter of nanoseconds.
Photocathodes
The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.
Recording of a beating heart of a zebrafish. Recorded at 2000 fps with the Lambert Instruments HiCAM on a fluorescence microscope. The blood cells were stained with a DS-red fluorophore.
monArch fluorescent voltage indicator observed with the Lambert Instruments HiCAM Fluo (1000 fps, 1200 x 1000 pixels).
SCAPE 2.0 dual-color, high-speed imaging of blood flow in a beating embryonic zebrafish heart acquired at 100 volumes per second over a 270 x 260 x 128 xyz micron field of view. The heart wall is shown in green (EGFP) and the red blood cells are shown in red (DsRed). Collaboration between Hillman Lab and Kimara Targoff.
Maximum resolution: 1280 x 1024 pixels
Frame rate:
1000 fps at full resolution
1500 fps at 1200 x 720 pixels
4000 fps at 640 x 480 pixels
7500 fps at 256 x 256 pixels
Pixel size: 6.6 um
Pixel depth: 8 or 12 bit
Minimum exposure time: 40 ns
Gating repetition rate: 100 kHz
Image intensifier: GaAsP (Others optional)
Computer interface: Streaming CoaXPress
Photon gain (max.): 36000 lm/m^2/lx
Maximum resolution: 1920 x 1080 pixels
Frame rate:
2000 fps at full resolution
2500 fps at 1280 x 960 pixels
5100 fps at 640 x 480 pixels
6400 fps at 512 x 384 pixels
Pixel size: 10 um
Pixel depth: 8 bit
Minimum exposure time: 40 ns
Gating repetition rate: 100 kHz
Image intensifier: GaAsP (Others optional)
Computer interface: Streaming CoaXPress 2.0
Photon gain (max.): 36000 lm/m^2/lx
Leonard Springerlaan 19
9727 KB Groningen
The Netherlands
Researchers around the world use the HiCAM Fluo in their studies. Opposite is an overview of the most recent publications describing research that was done using a HiCAM Fluo.
For a complete overview of applications, please visit our applications pages.
Imaging the voltage of neurons distributed across entire brains of larval zebrafish – December 16, 2023
Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection – February 22, 2023
High-speed, high-content volumetric microscopy with sub-cellular resolution applied to cell-identity resolved C. elegans – April 24. 2022
Photon-Sparse, Poisson Light-Sheet Microscopy – September 20, 2021
Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0 – Sep 27, 2019
Programmable Functionalization of Surfactant‐Stabilized Microfluidic Droplets via DNA‐Tags – April 8, 2019
5th floor,
Leonard Springerlaan 19
9727KB Groningen
The Netherlands
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